Cytometry - Revision history

Michael: /* Common markers */

2017-03-05T16:33:12Z

Common markers

← Older revision		Revision as of 16:33, 5 March 2017
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	==Common markers==		==Common markers==
	*CD3, CD4, CD8, CD5, CD7.		*[[CD3]], CD4, CD8, [[CD5]], CD7.
	*CD19, CD20, FMC7.		*CD19, [[CD20]], FMC7.
	*Kappa, lambda.		*Kappa, lambda.

Michael: /* Sample report */

2014-09-13T08:07:14Z

Sample report

← Older revision		Revision as of 08:07, 13 September 2014
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	NK-Cell Associated Markers		NK-Cell Associated Markers
	*CD56.		*[[CD56]].
	*CD16.		*CD16.

Michael at 20:13, 27 December 2013

2013-12-27T20:13:54Z

← Older revision		Revision as of 20:13, 27 December 2013
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	**B cell marker.		**B cell marker.
	**Should be ~=CD20.		**Should be ~=CD20.
	*CD23.		*[[CD23]].
	**+ve in [[chronic lymphocytic leukemia\|CLL/SLL]].		**+ve in [[chronic lymphocytic leukemia\|CLL/SLL]].
	*FMC7		*FMC7

Michael: w

2012-12-27T11:12:27Z

← Older revision		Revision as of 11:12, 27 December 2012
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	==Abnormal==		==Abnormal==
	*CD4>CD8 predominance classically seen in:		*CD4>CD8 predominance classically seen in:
	**Sarcoidosis.		**[[Sarcoidosis]].
	**Hodgkin's lymphoma.		**[[Hodgkin's lymphoma]].
	*T cell lymphoma - loss of one of the T-cell markers (CD2, CD3, CD5 or CD7).		*T cell lymphoma - loss of one of the T-cell markers (CD2, CD3, CD5 or CD7).
	**CD7 is usually the first to be lost.		**CD7 is usually the first to be lost.
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	*CD10.		*CD10.
	**Germinal centre marker.		**Germinal centre marker.
	**+ve in: follicular l., Burkitt l., precursor lymphoblastic leukemia.		**+ve in: [[follicular lymphoma]], [[Burkitt lymphoma]], precursor lymphoblastic leukemia.
	*CD19.		*CD19.
	**B cell marker.		**B cell marker.
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	**Should be ~=CD20.		**Should be ~=CD20.
	*CD23.		*CD23.
	**+ve in CLL/SLL.		**+ve in [[chronic lymphocytic leukemia\|CLL/SLL]].
	*FMC7		*FMC7
	**B cell marker.		**B cell marker.

Michael at 04:35, 8 February 2012

2012-02-08T04:35:31Z

← Older revision		Revision as of 04:35, 8 February 2012
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	Miscellaneous Markers		Miscellaneous Markers
	*CD11c.		*CD11c -- usu. +ve in [[marginal zone lymphoma]].
	*CD14.		*CD14.

Michael: /* Normal */

2010-08-11T20:58:47Z

Normal

← Older revision		Revision as of 20:58, 11 August 2010
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	*In reactive nodes T-cells predominate.		*In reactive nodes T-cells predominate.
	*Normal thymic tissue has cells that are positive for both CD4 and CD8.		*Normal thymic tissue has cells that are positive for both CD4 and CD8.
	*Kappa and lambda are not expressed by the same cell.		*Kappa (k) and lambda (l) are not expressed by the same cell.
	*Rule-of-thumb for normal k:l range is: <6:1 and 1:<3.<ref>SB. March 10, 2010.</ref>		*Rule-of-thumb for normal k:l range is: <6:1 and 1:<3.<ref>SB. March 10, 2010.</ref>
	**Lambda dominance is less common.		**Lambda dominance is less common.

	GS guidelines - non-malignant is:<ref>GS. LSC Procedure. March 11, 2010.</ref>		GS guidelines - non-malignant is:<ref>GS. LSC Procedure. March 11, 2010.</ref>
	*CD19 ~= CD20		*CD19 ~= CD20.
	*CD5 = CD3		*CD5 = CD3.
	*CD2 > CD3 and CD5		*CD2 > CD3 and CD5.
	*CD4 + CD8 ~= CD3		*CD4 + CD8 ~= CD3.
	*CD7 = the smallest number of T-cell		*CD7 = the smallest number of T-cell.

	==Abnormal==		==Abnormal==

Michael: /* Common markers= */

2010-08-11T20:56:31Z

Common markers=

← Older revision		Revision as of 20:56, 11 August 2010
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	#Laser scanning cytometry (LSC).		#Laser scanning cytometry (LSC).

	==Common markers===		==Common markers==
	*CD3, CD4, CD8, CD5, CD7.		*CD3, CD4, CD8, CD5, CD7.
	*CD19, CD20, FMC7.		*CD19, CD20, FMC7.

Michael: create

2010-08-11T20:56:17Z

create

New page

'''Cytometry''' is the quantification and measurement of cells.

It is typically done in the context of [[lymphoma]] via one of two techniques:
#[[Flow cytometry]].
#Laser scanning cytometry (LSC).

==Common markers===
*CD3, CD4, CD8, CD5, CD7.
*CD19, CD20, FMC7.
*Kappa, lambda.

==Normal==
*T-cells to B-cells usually 1:1.
*In reactive nodes T-cells predominate.
*Normal thymic tissue has cells that are positive for both CD4 and CD8.
*Kappa and lambda are not expressed by the same cell.
*Rule-of-thumb for normal k:l range is: <6:1 and 1:<3.<ref>SB. March 10, 2010.</ref>
**Lambda dominance is less common.

GS guidelines - non-malignant is:<ref>GS. LSC Procedure. March 11, 2010.</ref>
*CD19 ~= CD20
*CD5 = CD3
*CD2 > CD3 and CD5
*CD4 + CD8 ~= CD3
*CD7 = the smallest number of T-cell

==Abnormal==
*CD4>CD8 predominance classically seen in:
**Sarcoidosis.
**Hodgkin's lymphoma.
*T cell lymphoma - loss of one of the T-cell markers (CD2, CD3, CD5 or CD7).
**CD7 is usually the first to be lost.

==Sample report==
B-Cell Associated Markers
*CD10.
**Germinal centre marker.
**+ve in: follicular l., Burkitt l., precursor lymphoblastic leukemia.
*CD19.
**B cell marker.
**Should be ~=CD20.
*CD20.
**B cell marker.
**Should be ~=CD20.
*CD23.
**+ve in CLL/SLL.
*FMC7
**B cell marker.
*Kappa+CD19/20+.
**B cell clonality.
*Lambda+CD19/20+
**B cell clonality.

T-Cell Associated Markers
*CD3.
**CD3 ~= CD4 + CD8.
**Usually: CD2 > CD3 > CD7.
*CD5.
**Usually: CD2 > CD5 > CD7.
*CD4.
**CD3 ~= CD4 + CD8.
*CD8.
**CD3 ~= CD4 + CD8.
*CD7.
**Usually least prevalent T cell marker; < CD2, < CD3, < CD5.
*CD2.
**Usually most prevalent T cell marker; > CD3, > CD5, > CD7.

NK-Cell Associated Markers
*CD56.
*CD16.

Miscellaneous Markers
*CD11c.
*CD14.

==See also==
*[[Haematopathology]].
*[[Flow cytometry]].
*[[Cytopathology]].

==References==
{{reflist|2}}

[[Category:Haematopathology]]